School of Medicine and Public Health
Human Proteomics Program
Equipment and Resources
Bruker SolariX XR FT-ICR Mass Spectrometer
The 12T SolariX XR FT-ICR MS is a high performance hybrid system which combines the unsurpassed FT-ICR technologies and advanced triple quadrupole (QqQ) into a single instrument. This instrument has unprecedented analytical power and versatility, ultra high-resolution and sensitivity, and a variety of tandem mass spectrometry (MS/MS) capabilities for top-down proteomics and metabolomics studies. The SolariX XR system allows for mass resolution greater than 10 million. This level of eXtreme Resolution (XR) represents an order of magnitude quantum jump in mass resolving power, opens up entirely new scientific frontiers in chemistry and molecular biology and enables novel scientific investigations. The solariX XR is capable to reveal the fine structure in isotopic patterns that are uniquely specific to the exact molecular formulae of the detected compounds.
For details, please visit Bruker website.
Bruker Impact II Q-TOF
Impact II Q-TOF is a bench-top time-of-flight mass spectrometer with ultra-high scan rate, mass accuracy and sensitivity for high-throughput proteomics and metabolomics analysis. The new Impact II is the latest innovation in Bruker's unique UHR-QqTOF (Ultra-High Resolution Qq-Time-Of-Flight) mass spectrometry product line with industry-leading > 50,000 Full-Sensitivity Resolution (FSR).
For details, please visit Bruker website.
Bruker maXis II Q-TOF
maXis II Q-TOF is a high resolution time-of-flight mass spectrometer with additional electron transfer dissociation feature for the analysis of large protein, protein complexes and PTMs. It provides high versatility from small molecules up to megadalton complexes.
For details, please visit Bruker website.
Waters ACQUITY UPLC M-Class System
The ACQUITY UPLC M-Class System delivers the most robust, reliable, and highest quality nano- to microscale UPLC separations to every laboratory performing LC/MS analyses. With unprecedented usability, the ACQUITY UPLC M-Class System enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even amid a complex matrix over several orders of magnitude. Confidently achieve aboratory goals with high-efficiency ACQUITY UPLC M-Class Columns ranging from 75 μm to 1 mm I.D. by using complementary mass spectrometry to support our full range of column I.D.s, or run 300 μm to 1 mm I.D. columns with optical detection. The instrument is capable of applying improved 2D approach that uses RP at pH 10 in the first dimension, followed by RP at pH 2 in the second dimension for results that far exceeds those of conventional IEX methodologies.
For details, please visit Waters website.
Waters ACQUITY UPLC H-Class System
The ACQUITY UPLC H-Class System is the first UPLC/UHPLC system that delivers the flexibility of quaternary solvent blending with the advanced performance expected of UPLC separations: high resolution, sensitivity and improved throughput. The system's holistic design is targeted for routine analysis and method development use and is perfectly suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations.
For details, please visit Waters website.
Waters nanoACQUITY UPLC System
The Waters nanoACQUITY UPLC system is designed for capillary-to-nano-scale separations. Its sensitivity, resolution, and reproducibility well suit it for biomarker discovery, metabolomics and proteomics applications, including metabolite/protein identification and characterization.
For details, please visit Waters website.
TriVersa™ NanoMate® and ESI Chip® System
TriVersa is an automated nanoelectrospray source that integrates LC/MS, fraction collection and chip-based infusion in one system. It is ideal for characterization for post-translational modification (PTM) and biomarker discovery.
Advion’s ESI Chip unleashes the power of MS through miniaturization. 
This microfluidics chip contains an array of nanoelectrospray nozzles, each one-fifth the diameter of a human hair, etched in a silicon wafer. The fabrication technology is highly reproducible to ensure the integrity of each nozzle.
For details, please visit the Advion web site.
Bruker timsTOF Pro
The timsTOF Pro with PASEF® technology delivers revolutionary improvements in scan speed, with enhanced specificity and high sensitivity. With PASEF technology, > 100 Hz sequencing speed can be achieved. Extremely high data acquisition speed increases the throughput of biological experiments significantly. Due to the sensitivity gains from PASEF, more peptides and their post-translational modifications (PTMs) can be identified. The unique instrument design with orthogonal reflection into TIMS enables routine operation over thousands of samples without instrument cleaning.
For details, please visit Bruker website.
Bruker nanoElute Nano-HPLC Chromatography System
The nanoElute is a high-performance nanoflow liquid chromatography system with an intuitive and flexible user interface, covering a broad range of applications from single cell to high throughput routine proteomics. This system provides users with the highest chroma-tographic resolution resulting in exceptional peptide identification and quantification.
For details, please visit Bruker website.
Agilent 6545XT AdvanceBio LC/Q-TOF
The Agilent 6545XT AdvanceBio LC/Q-TOF system is designed to handle multiple workflows in biopharmaceutical characterization. When users need to access the most information at the intact protein level, automatically confirm a sequence through peptide mapping, and confidently understand PTMs, Agilent provides a UHPLC Q-TOF that delivers. Accurately profile major and minor proteoforms with time-of-flight technology, offering new levels of sensitivity and clarity to intact protein mass spectrometry. Easily dig deeper into peptide digests with the Iterative MS/MS mode of operation.
For details, please visit Agilent website.
Agilent 1290 Infinity II 2D-LC
Increase resolution where you need it with the 1290 Infinity II 2D-LC System, which offers comprehensive two-dimensional liquid chromatography (2D-LC), (multiple) heart-cutting 2D-LC analysis, and high-resolution sampling. This system allows you to use 2D-HPLC for making buffer separations compatible to MS detection, while easy-to-use software enables fast method setup and refined data analysis.
For details, please visit Agilent website.